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pseap 2 control dna  (TaKaRa)


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    Structured Review

    TaKaRa pseap 2 control dna
    Ionic interaction analysis of mAb C and DNA. 0.3 μg of Sal I-linearized <t>pSEAP-2</t> control DNA was mixed with water, medium or mAb C for 40 minutes at room temperature with occasional mixing and then subjected to 1% agarose gel electrophoresis. Lane 1: DNA in dH 2 O; lane 2 and 8: DNA in Modified Tyrode's medium without BSA (MTM); lane 3: DNA plus 0.1 μg mAb C in MTM; lane 4: DNA plus 0.3 μg mAb C in MTM; lane 5: DNA plus 1 μg mAb C in MTM; lane 6: DNA plus 3 μg mAb C in MTM; and lane 7: DNA plus 10 μg mAb C in MTM.
    Pseap 2 Control Dna, supplied by TaKaRa, used in various techniques. Bioz Stars score: 95/100, based on 289 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/pseap+2+control+dna/pmc00113740-231-25-28?v=TaKaRa
    Average 95 stars, based on 289 article reviews
    pseap 2 control dna - by Bioz Stars, 2026-08
    95/100 stars

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    1) Product Images from "Effective generation of transgenic pigs and mice by linker based sperm-mediated gene transfer."

    Article Title: Effective generation of transgenic pigs and mice by linker based sperm-mediated gene transfer.

    Journal: BMC Biotechnology

    doi: 10.1186/1472-6750-2-5

    Ionic interaction analysis of mAb C and DNA. 0.3 μg of Sal I-linearized pSEAP-2 control DNA was mixed with water, medium or mAb C for 40 minutes at room temperature with occasional mixing and then subjected to 1% agarose gel electrophoresis. Lane 1: DNA in dH 2 O; lane 2 and 8: DNA in Modified Tyrode's medium without BSA (MTM); lane 3: DNA plus 0.1 μg mAb C in MTM; lane 4: DNA plus 0.3 μg mAb C in MTM; lane 5: DNA plus 1 μg mAb C in MTM; lane 6: DNA plus 3 μg mAb C in MTM; and lane 7: DNA plus 10 μg mAb C in MTM.
    Figure Legend Snippet: Ionic interaction analysis of mAb C and DNA. 0.3 μg of Sal I-linearized pSEAP-2 control DNA was mixed with water, medium or mAb C for 40 minutes at room temperature with occasional mixing and then subjected to 1% agarose gel electrophoresis. Lane 1: DNA in dH 2 O; lane 2 and 8: DNA in Modified Tyrode's medium without BSA (MTM); lane 3: DNA plus 0.1 μg mAb C in MTM; lane 4: DNA plus 0.3 μg mAb C in MTM; lane 5: DNA plus 1 μg mAb C in MTM; lane 6: DNA plus 3 μg mAb C in MTM; and lane 7: DNA plus 10 μg mAb C in MTM.

    Techniques Used: Agarose Gel Electrophoresis, Modification

    Genotype analysis of transgenic mice. Southern blot analysis of transgenic mice from F1 generation. The genomic DNA was isolated from 6-week-old mouse-tails and digested with Bgl I. The 1.3 kb Bgl I fragment of pSEAP-2 control DNA was used as a probe. The numbers in bold indicate positive detection on the blot.
    Figure Legend Snippet: Genotype analysis of transgenic mice. Southern blot analysis of transgenic mice from F1 generation. The genomic DNA was isolated from 6-week-old mouse-tails and digested with Bgl I. The 1.3 kb Bgl I fragment of pSEAP-2 control DNA was used as a probe. The numbers in bold indicate positive detection on the blot.

    Techniques Used: Transgenic Assay, Southern Blot, Isolation



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    pseap 2 control dna  (TaKaRa)
    95
    TaKaRa pseap 2 control dna
    Ionic interaction analysis of mAb C and DNA. 0.3 μg of Sal I-linearized <t>pSEAP-2</t> control DNA was mixed with water, medium or mAb C for 40 minutes at room temperature with occasional mixing and then subjected to 1% agarose gel electrophoresis. Lane 1: DNA in dH 2 O; lane 2 and 8: DNA in Modified Tyrode's medium without BSA (MTM); lane 3: DNA plus 0.1 μg mAb C in MTM; lane 4: DNA plus 0.3 μg mAb C in MTM; lane 5: DNA plus 1 μg mAb C in MTM; lane 6: DNA plus 3 μg mAb C in MTM; and lane 7: DNA plus 10 μg mAb C in MTM.
    Pseap 2 Control Dna, supplied by TaKaRa, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/pseap+2+control+dna/pmc00113740-231-25-28?v=TaKaRa
    Average 95 stars, based on 1 article reviews
    pseap 2 control dna - by Bioz Stars, 2026-08
    95/100 stars
    		  Buy from Supplier

    Image Search Results


    Ionic interaction analysis of mAb C and DNA. 0.3 μg of Sal I-linearized pSEAP-2 control DNA was mixed with water, medium or mAb C for 40 minutes at room temperature with occasional mixing and then subjected to 1% agarose gel electrophoresis. Lane 1: DNA in dH 2 O; lane 2 and 8: DNA in Modified Tyrode's medium without BSA (MTM); lane 3: DNA plus 0.1 μg mAb C in MTM; lane 4: DNA plus 0.3 μg mAb C in MTM; lane 5: DNA plus 1 μg mAb C in MTM; lane 6: DNA plus 3 μg mAb C in MTM; and lane 7: DNA plus 10 μg mAb C in MTM.

    Journal: BMC Biotechnology

    Article Title: Effective generation of transgenic pigs and mice by linker based sperm-mediated gene transfer.

    doi: 10.1186/1472-6750-2-5

    Figure Lengend Snippet: Ionic interaction analysis of mAb C and DNA. 0.3 μg of Sal I-linearized pSEAP-2 control DNA was mixed with water, medium or mAb C for 40 minutes at room temperature with occasional mixing and then subjected to 1% agarose gel electrophoresis. Lane 1: DNA in dH 2 O; lane 2 and 8: DNA in Modified Tyrode's medium without BSA (MTM); lane 3: DNA plus 0.1 μg mAb C in MTM; lane 4: DNA plus 0.3 μg mAb C in MTM; lane 5: DNA plus 1 μg mAb C in MTM; lane 6: DNA plus 3 μg mAb C in MTM; and lane 7: DNA plus 10 μg mAb C in MTM.

    Article Snippet: After washing twice with centrifugation at 3000 rpm for 1.5 min, the sperm pellet was resuspended in incubation buffer containing 500 ng of 32 P-labeled pSEAP-2 control DNA (Clontech Laboratories Inc., Palo Alto, CA; catalog #6052–1, vector map available at Clontech web site) made by T 4 DNA polymerase, and incubated for another 40 min. After washing the sperm twice with incubation buffer to remove unbound 32 P-labeled DNA, samples were measured by liquid scintillation counting.

    Techniques: Agarose Gel Electrophoresis, Modification

    Genotype analysis of transgenic mice. Southern blot analysis of transgenic mice from F1 generation. The genomic DNA was isolated from 6-week-old mouse-tails and digested with Bgl I. The 1.3 kb Bgl I fragment of pSEAP-2 control DNA was used as a probe. The numbers in bold indicate positive detection on the blot.

    Journal: BMC Biotechnology

    Article Title: Effective generation of transgenic pigs and mice by linker based sperm-mediated gene transfer.

    doi: 10.1186/1472-6750-2-5

    Figure Lengend Snippet: Genotype analysis of transgenic mice. Southern blot analysis of transgenic mice from F1 generation. The genomic DNA was isolated from 6-week-old mouse-tails and digested with Bgl I. The 1.3 kb Bgl I fragment of pSEAP-2 control DNA was used as a probe. The numbers in bold indicate positive detection on the blot.

    Article Snippet: After washing twice with centrifugation at 3000 rpm for 1.5 min, the sperm pellet was resuspended in incubation buffer containing 500 ng of 32 P-labeled pSEAP-2 control DNA (Clontech Laboratories Inc., Palo Alto, CA; catalog #6052–1, vector map available at Clontech web site) made by T 4 DNA polymerase, and incubated for another 40 min. After washing the sperm twice with incubation buffer to remove unbound 32 P-labeled DNA, samples were measured by liquid scintillation counting.

    Techniques: Transgenic Assay, Southern Blot, Isolation